Last edited by Kazizahn
Friday, August 14, 2020 | History

4 edition of Virus inactivation by ozone found in the catalog.

Virus inactivation by ozone

Dena Adachi

Virus inactivation by ozone

by Dena Adachi

  • 143 Want to read
  • 31 Currently reading

Published by National Library of Canada in Ottawa .
Written in English


Edition Notes

Thesis (M.Sc.) -- University of Toronto, 2001.

SeriesCanadian theses = -- Thèses canadiennes
The Physical Object
FormatMicroform
Pagination2 microfiches : negative. --
ID Numbers
Open LibraryOL19508443M
ISBN 100612586901
OCLC/WorldCa52575159

Comparison of% virus inactivation times in the pre-sent study with those from a recent study with chlorine revealed similarities betweeninactivationofSAandHRV by chlorine and SA inactivation by ozone. Considerably lower values derived from HRV-ozone data demonstrated the superiority of ozone in the inactivation of the human strain. Inactivation (disinfection) processes 47 Figure Disinfection (2-log) of microorganisms by free available chlorine (White, ). Table Disinfection time–chlorine concentration envelopes for 99% virus inactivation at 0–5°C and 10°C CT in mg/min l–1 pH range 0–5°C 10°C – 12 8 – 20 15 – 30

  virus. in 30 s. at a minimal final concentration of. at least. 30 %. Alcohol constitutes the. basis for many hand rubs routinely used in health care settings. Ou. r. findings are. therefore. of utmost importance in the current outbreak situation to. minimize viral. transmission and maximize virus. inactivation.   Since ozone has been proven to kill percent of pathogens in the air, including SARS Coronavirus and influenzas such as H5N1, researchers .

The inactivation of simian rotavirus Sa and human rotavirus type 2 (Wa) by ozone was compared at 4/sup 0/C by using single-particle virus stocks. Although the human strain was clearly more sensitive, both virus types were rapidly inactivated by ozone concentrations of mg/liter or greater at . Ct values; concentration of ozone multiplied by contact time with virus; were determined from application of the efficiency factor hom (EFH) model. Ct values for 4-log (%) ozone inactivation at 5°C and pH 7 ranged from to mg/l min for AD40 and.


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Virus inactivation by ozone by Dena Adachi Download PDF EPUB FB2

Virus reductions by Virus inactivation by ozone book were determined using a dose of mg of ozone/liter at pH 7 and 5°C for up to 5 min. Based on two RT-PCR assays, the reductions of Norwalk virus were >3 log10 within. The ozone dose for 99% inactivation was two times higher than for 90% inactivation.

The required ozone concentration at 85% RH was lower than at 55% RH. In summary, ozone should be an effective method for reducing the viral number between 1 and 3 logs on by: radical species formed when ozone decom-poses (Kim, Yousef, & Chism, ). Addi-tionally, ozone could react with amino acids, proteins, protein functional groups, and nu-cleic acids very rapidly (Langlais, Reckhow, & Brink, ), causing virus inactivation.

Therefore, viruses could be inactivated by ozone acting on the protein structure of a. This study was designed to test the efficacy of an air treatment using ozone and relative humidity (RH) for the inactivation of airborne viruses. Four phages (φX, PR, MS2 and φ6) and one eukaryotic virus (murine norovirus MNV-1) were exposed to low ozone concentrations ( ppm for phages and ppm for MNV-1) and various levels of RH for 10 to 70 by: 1.

Ozone is an effective disinfectants against all types of waterborne pathogens, including viruses. While ozone has typically been used to treat drinking water, its application to wastewater (WW) has also been tested.

However, in particular for virus inactivation, data regarding its Author: Camille Wolf, Urs von Gunten, Tamar Kohn. Roy et al. reported that ozone damages two of the four polypeptide chains present in the viral capsid of PV1; however, protein coat alteration does not significantly impair the adsorption of the virus or alter the integrity of viral particles; damage to viral nucleic acids by ozone was the foremost reason for PV1 inactivation[18].

The mechanism of enteroviral inactivation by ozone was investigated with poliovirus 1 (Mahoney) as the model virus. Ozone was observed to alter two of the four polypeptide chains present in the viral protein coat of poliovirus 1. However, the alteration of the protein coat did not significantly impair virus adsorption or alter the integrity of.

A comparison of the effect of gaseous ozone on murine norovirus (MNV-1) and hepatitis A virus (HAV) adsorbed on fresh raspberries was performed. Infectious MNV-1 was highly inactivated (> log 10) by ozone (3 ppm, 1 min). The raspberry matrix seems to enhance inactivation by ozone.

The inactivation of viral particles by ozone may take place by a variety of mechanisms which range from direct physico-chemical effects to more indirect immunological pathways. Virions coated by a lipid glycoprotein envelope such as rectoviruses, hepatitis B and C, Herpes 1 and 2, and Epstein-Barr among others, are vulnerable to the influence.

Ozone is a highly oxidizing gas easily generated from atmospheric oxygen with inexpensive equipment and is commonly used for the disinfection of municipal water, foods, and surfaces. We report tests of the ability of ozone to inactivate enveloped respiratory viruses (influenza A virus and respiratory syncytial virus), chosen as more easily handled surrogates for SARS-CoV-2, on N95.

page 1 Enveloped Virus Inactivation on Personal Protective Equipment by Exposure to Ozone Emmeline L. Blanchard,a bJustin D. Lawrence, Jeffery bA. Noble,c Minghao Xu,d Taekyu Joo, Nga Lee Ng,b,e,f Britney E. Schmidt,b dPhilip J. Santangelo,a M.G. Finn,g * aWallace H.

Coulter Department of Biomedical Engineering, bSchool of Earth and Atmospheric Sciences. Inactivation of Influenza Virus by Ozone Gas More than % of influenza A virus particles attached to a plastic carrier were inactivated by exposure to 10 ppm (V/V) - ozone gas for min at 23 to 29℃and a relative humidity of 64 to 65%.

When the virus was exposed to 20 ppm (V/V) - ozone gas for min, more than % was inactivated. Ozone is much more effective against viruses and bacteria than it is against protozoa, therefore, if protozoa inactivation is an objective, any virus and/or bacteria inactivation objectives will be incidentally achieved by meeting protozoa inactivation objectives.

In such a case, the validation study will be driven by the. Vesicular stomatitis virus, encephalomyocarditis virus, GDVII virus, Staphylococcus aureus, Pseudomonas fluorescens, Salmonella typhimurium, enteropathogenic Escherichia coli, Vibrio cholerae, and Shigella flexneri were inactivated by treatment with ozone.

When microorganisms were suspended in phosphate-buffered saline, they were inactivated. When the virus was exposed to 20 ppm (V/V) – ozone gas for min, more than % was inactivated. In contrast, the virus remained active after 10 hours under similar conditions without ozone gas. These data suggest that office disinfection against inf luenza viruses might be accomplished by ozone gas fumigation during night-time hours.

Virus inactivation treatments were made by using acetic acid (CH 3 COOH), hydrogen peroxide (H 2 O 2), hydrochloric acid (HCl), sodium hypochlorite (NaClO), Triton Xdry heat, heated water, ozone (O 3), and UV ( nm wavelength).

The most effective treatments for reducing virus concentration were HCl, heated water (65 °C) and ozone (10 g.

Additionally, ozone could react with amino acids, proteins, protein functional groups, and nucleic acids very rapidly (Langlais, Reckhow, & Brink, ), causing virus inactivation. Therefore, viruses could be inactivated by ozone acting on the protein structure of a virus.

Evaluation of inactivation effect of ozonized water against ASFV. WT-ASFV or reporter ASFV (1010 and 10 TCID 50 /mL) were incubated at room temperature (20−25 °C) with different concentrations of ozonized water (5, 10 and 20 mg/L) (, v/v), respectively, for 1, 3, 6 or 10 min. Then neutralizer (, v/v) was added.

After min incubation at room temperature, PAMs (in. Ozone generators are able to make ozone from normal air and are normally used as room disinfectants. The antipathogenic effects of ozone have been substantiated for several decades. Its killing action upon bacteria, viruses, fungi, and in many species of protozoa, serve as the basis for its increasing use in disinfecting municipal water.

During the SARS epidemic ofozone sterilization was successfully used to purify environments infected with the deadly Coronavirus, SARS-CoV-1, the virus which cause s the SARS diseas e. As SARS-Cov-1 is also a member of the Coronavirus family, it is highly likely that ozone sterilization would be effective at killing SARS-CoV -2, the.

It is also shown that acidic ozone water with a p H value of 4 or less is very effective means of virus inactivation if provided in conjunction with an ozone concentration of 20 mg/l or higher. The new flu known as swine flu, appearing almost daily in the news as of the summer ofis a highly contagious disease.It was observed that the survival fraction of airborne virus decreased exponentially with increasing ozone dose.

Airborne viruses required ozone doses of to and to min-mg/m 3 for 90% and 99% inactivation, respectively. For all four tested, the ozone dose for 99% inactivation was 2 times higher than that for 90% inactivation.The inactivation of both virus types was moderate at neutral pH, and the sensitivities to chlorine dioxide were similar.

The observed enhancement of virucidal efficiency with increasing pH was contrary to earlier findings with chlorine- and ozone-treated rotavirus particles, where efficiencies decreased with .